Pykhtyeyeva O. Biocatalytic Ensembles on the Base of the Proteolytic Enzyme Incorporated into the Various Liotropic Mesophases

. Subject of investigation: supramolecular compounds based on a-chymotrypsin incorporated into various liotropic mesophases. The aim of investigation: working out and optimization of the conditions of synthesis, investigation of properties and structure of the biocatalytic ensembles on a basis of surfactants and a-chymotrypsin. Methods of investigation and appliances: IR spectroscopy, spectrophotometry, nephelometry, NMR spectroscopy (13C NMR relaxometry, hydrophilic paramagnetic probing, measurements of the self-diffusion). Theoretical results and their novelty: It was shown that enzyme was incorporated into inner aqueous cavity of the reversed cylindrical micelle from Brij-96, keeping own activity. Established that activity, thermostability and position of the pH-optimum for the a-chymotrypsin containing nanocapsules made from poly-N,N-didodecyl-N,N-diallylammonium bromide depended on concentration and type of surfactant which formed the outer monolayer. Established that incorporation of a-chymotryps in into colloid structures led to increase of their stability by coalescence of nanocapsules, or syneresis of cryogels. Practical results and novelty: The possibility of obtaining of the catalytic system on a basis of the a-chymotrypsin and surfactant Brij-96, polymerized nanocapsules based on N,N-didodecyl-N,N-diallylammonium bromide and polyvinyl alcohol (PVA) cryogel by addition of DMSO has been proposed. It was shown that nano-encapsulated a-chymotrypsin kept own activity and had high thermostability in both the aqueous and organic media. It was shown that this catalyst kept own activity after more then ten utilization cycles, and after storage in frozen state for three months. Subject and rate of introducing: papers, presentations on the scientific conferences, utilization of the results of work by investigations and teaching in universities, colleges and institutes of Ukraine. Effectiveness of introducing: is provided for the use of the high technological method of immobilizing the water-soluble enzymes . Field of application: chemistry, biotechnology and medicine.