Poronnik O. Obtaining and characterization of the novel highly productive strain of Arnebia euchroma (Royle) Jonst. cultured cells.

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0401U001644

Thesis Registration Form

0401U001644.pdf

Applicant for

Specialization

  • 03.00.20 - Біотехнологія

29-05-2001

Specialized Academic Board

Д 26.237.01

Institute of Molecular Biology and Genetics of NAS of Ukraine

Essay

Through the cell selection technique novel highly productive strain of Arnebia euchroma (Royle) Jonst. cu-ltured cells has been obtained. If maintained as a suspension culture it may accumulate up to 8% of shikonin per dry tissue, whose yield would amount to 16-18 g/l for 14 days of growth, or 1.12 g of shikonin per 1 l of suspension for passage. The phenotypic heterogeneity of AE-2 strain was found to be mainly due to hereditary heterogeneity (coefficient of inheritance h2= +0.82), while that of in AE-3 strain, electivelyderived from it, resulted from paratypical variability (h2=+007). Growth and biosynthetic characteristics of the novel AE-3 strain allow to recommend it as an object for the biotechnological shikonin production.

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