Lymans'ka O. Design of molecular genetic test-systems for early detection of bovine leukemia virus and preimplantation embryo sexing

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0401U001804

Thesis Registration Form

0401U001804.pdf

Applicant for

Specialization

  • 03.00.20 - Біотехнологія

29-05-2001

Specialized Academic Board

Д 26.237.01

Institute of Molecular Biology and Genetics of NAS of Ukraine

Essay

New primer sets for early bovine leukemia virus (BLV) detection are designed on the basis multiple alignment of gene env sequences of different BLV isolates from EMBL/ GenBank and following thermody- namical analysis of primers and amplicons. Theoretical and experi- mental comparison of designed and known primer sets for BLV de- tection is carried out. It is shown designed primers have higher spe- cificity and accuracy than known primers. Main principles of polyme- rase chain reaction (PCR) test-system characterization and primers design are modified. It is shown primers homology degree is a key parameter for PCR accuracy. The universal primer system for preim- plantational cattle, sheep, goat and pig embryo sexing is created.

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