Ostankov M. Theoretical substantiation and experimental checking of the efficiency of rapid two-stage freezing

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0402U000868

Applicant for

Specialization

  • 03.00.19 - Кріобіологія

26-02-2002

Specialized Academic Board

Д 64 242.01

Essay

Rapid two-stage freezing in comparison with a linear one provides a high resistance of being preserved mice bone marrow cells to posthypertonic lysis, ice crystals formation in separate cells. It significantly reduces the duration and labour-intensiveness of freezing-thawing procedure and provides the integrity of three main lineage of hemopoiesis of bone marrow cells. Much more simple apparatuses are needed to apply this method in comparison with those for freezing with constant cooling rate.

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