Kravchenko S. Allelic polymorphisms of minisatellite and microsatellite loci among different populations from Ukraine

The object of study were human polymorphic DNA-loci, subject - allele, genotype and haplotype frequencies of these loci . The methods have been used: DNA isolations and purification, polymeraze chain reaction, different type of electrophoresys, statistical analysis. Allele, genotype and haplotype frequencies data in populations under investigations have been obtained. No genetic differences were observed among populations from Kiev, Lviv, Kremenchuk and Alchevsk. The allele and haplotype diversity values of Y-chromosome loci in Slavic origin populations from Ukraine, Russia and Belorussia as well as in Crimea tatars population have been calculated. It has been revealed that Crimea tatars population to be the most heterogeneous among all others under investigation. For Ukraine, Russia and Belorussia populations the network of most common haplotypes has been constructed. It has been shown that two haplotypes (13/11/11/24/16 and 13/11/10/25/16) are major and most probably are an ancestral haplotypes in Eastern Slavic populations. Calculated genetic distances based on Y-chromosome allelic polymorphisms data demonstrate that three Eastern Slavic populations are genetically close to one another and remote from European and Asian populations. Kit of informative DNA-markers has been proposed for forensic analysis in Ukraine.