Balashova I. Marking ofTriticum aestivum Vrn and Ppd genes the methods of PCR-analysis.

The thesis contains theoretical and experimental material concerning using PCR- analysis for investigating molecular-genetic polymorphism of wheat. The using the various methods of analysis and vast genetic material PCR-markers to genes Vrn-B1, Ppd-B1a, Ppd-D1a were produced and marker system allowing us to detect the gene Vrn-D1 alleles was formed. The close linkage between SBC 780 and SBC 350 markers and genes Vrn-D1 and Ppd-D1a, respectively, was the determined. Analysis of nucleotide sequence of the SBC 657 marker by a number of computer programmes allows us to attribute the sequenced fragment to transcribed part of bread wheat DNA. The probability of coding protein molecule of size in 218 aminoacids is predicted. The high level of homoeology between the bread wheat genes Vrn-B1 and Vrn 4 as well as bread wheat gene Ppd-B1a and the gene for sensitivity to photoperiod in barley was observed. The possibility of use markers developed for identification of genotypes as to the Vrn-D1, Vrn-B1, Ppd-D1a, Ppd-B1a genes in the bread wheat varieties and lines of various origin was shown