Rebriyev A. The development of biosensors based on photopolymerizable compositions of monomer- oligomeric type

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0403U000628

Applicant for

Specialization

  • 03.00.20 - Біотехнологія

24-02-2003

Specialized Academic Board

Д 26.240.01

Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine

Essay

The object of investigation - enzymatic biosensors, photopolymerizable compositions of monomer-oligomeric type (FPC). The aim - creation of effective and simple in manufacturing electrochemical biosensors with use FPC as immobilization matrix. Methods: potentiometric, IR- spectroscopy, photocolorimetric. The selection of FPC for immobilization of enzymes was carry out. The influence of immobilization on activity of enzymes was investigated and optimum immobilization conditions were determined. The developed biosensors for assay of glucose and urea, and their properties were investigated. Results can be used for industrial manufacturing biosensors. Field of application: scientic and educational institutes of medical and biological type.

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