Litovkin K. Genotypic features of morphogenic reactions in barley tissue culture

Research subject: barley immature embryo and anther culture. Research objectives: search for experimental ways to improve the efficiency of barley regenerants production technologies, determination of the role of genotype in processes of callus formation and plant regeneration. Methods: in vitro culture, light microscopy, genetic analysis and variational statistics. Theoretical and practical results: conditions of morphogenic callus formation and plant regeneration in spring barley immature embryo and isolated scutellar tissue culture have been improved. Optimum conditions of anther pretreatment are found. Detection of correlation between regeneration capacity in vitro and some field traits of barley plants allow to forecast tissue culture response of different genotypes. Novelty of introduced: the influence of some marker genes on barley immature embryo and anther culture response has been studied for the first time. The influence of the mutation in the barley homeobox-gene on regeneration capacity inimmature embryo culture has been detected for the first time. Never before a correlation between regeneration ability in immature embryo culture and some field traits of plants has been detected. A study of the genetic control of regeneration capacity under proposed immature embryo culture conditions has been performed for the first time. Degree of implementation: the results of the investigation have been included in a course of Agricultural biotechnology. Efficiency: in vitro technologies of barley breeding will be improved. Sphere of implementation: biotechnology, genetics, agriculture. Key words: Hordeum vulgare L., morphogenesis, immature embryo culture, anther culture, genetic analysis.