Tarasov A. Sensitivity of Human Fetal Liver Hematopoietic Progenitor Cells to the Effects of Low Temperature Preservation

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0403U002170

Applicant for

Specialization

  • 03.00.19 - Кріобіологія

17-06-2003

Specialized Academic Board

Д64.242.01

Essay

The thesis is dedicated to the problem of preservation of human fetal liver hematopoietic progenitor cells (HFL HPCs). To this end, the immunophenotype, the viability, the colony-forming activity of the cryopreserved HFL HPCs, the osmotic reaction of the isolated HPC, and the biophysical characteristics of the mass transfer across the membrane of HPC were studied. The content of the HPCs in HFL was shown to make about 1%, the majority of the HFL cells belonging to the erythroid lineage. The HFL HPCs possess high colony-forming activity, more than 60% of them generate hematopoietic colonies in semi-solid medium, the colony-forming potential decreasing with the gestation age of the sample. The HFL HPCs were demonstrated to be more labile to the effect of the convenient cryopreservation than the erythroid HFL progenitors, which testifies to the necessity of the optimization of the HFL preservation protocol. The osmotic reaction of the CD34+CD38- HFL HPC is featured by high values of the osmotically inactive volume and activation energy for DMSO (conveniently used as a cryoprotectant) transport. The results obtained can be used in the medical practice as well as in the development of the cryopreservation protocls for HSC.

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