Samchenko I. Influence of cryopreservation and conditions of processing on a functional condition of xenografts and adrenal gland organ cultures in vitro.

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0403U002372

Thesis Registration Form

0403U002372.pdf

Applicant for

Specialization

  • 03.00.19 - Кріобіологія

17-06-2003

Specialized Academic Board

Д 64.242.01

Institute of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine

Essay

In the work the comparative analysis of morphofunctional characteristics of native and cryopreserved adrenal gland organ cultures of newborn piglets under condition of their studying in vitro (reculture) and in vivo at transplantation to adrenal-ectomized male of white rats is carried out. Also investigated influence of modificators of steroidogenesis and secretion (chloride of cadmium, colchicines, an extract of a hypophysis) on functional and histological characteristics of organ culture in vitro and in vivo. The conclusion about expediency of use of cryopreserved endocrine material as xenograft is made. Key words: cryopreservation, adrenal organ culture, xenotransplantation, basal and stimulated secretion of glucocorticoid

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