Zakharchuk N. Phytotoxic metabolites of Phytophthora infestans and their using in creation of the resistance forms of potato

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0403U002763

Applicant for

Specialization

  • 06.01.11 - Фітопатологія

27-06-2003

Specialized Academic Board

Д 26.004.02

National University of Life and Environmental Sciences of Ukraine

Essay

Object - fungus Ph. infestans, its metabolites and variety of a potato with different resistance to late blight. Methods - phytopathologycal, physico-chemical, biotechnological, selection. The novelty - is designed a technique of allocation and clearing phytotoxic of metabolites Ph. infestans, which one bases on cultivation of pathogen in a fluid medium Henninger during 18 day (beginning of a fixed phase) and deposition of metabolites by ammonium sulphate (0,7 saturations) with the subsequent gel - fractionating on "Sephadex" G-75. PTM Ph. infestans, used as the selective factor in concentrations 0,5-5,0 mg/mls, are capable to improve stability of plants of a potato to late blight. Results - the prospects of usage of methods of cell-like selection with PTM is justified Ph. infestans as than the selective factors, for creation of breeding material and varieties of a potato with heightened (on 2-4 balls is higher basic varieties) immunity to pathogen in a complex with other useful tags and engaging it in selection practice. Branch - phytopathology, an agriculture, breeding.

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