Borysiyk M. The stady at the activity of proteolytic ferments of pancreatic and non-pancreatic origin in the dynamics of the experimental diabetes mellitus

The thesis is devoted to the study of the changes of the activity of important proteolytic systems and their role in arising vascular complications in case of experimental diabetes mellitus. Activity of elastase and its inhibitors (alpha-1 proteinase inhibitor, alpha-2-macroglobulin and acid resistant inhibitors), thrombin and its inhibitors (alpha-2- macroglobulin) in case of streptozotocine and aloxane models of diabetes in rats in blood serum, aorta tissues and pancreas were determined and compared in the thesis. The results of this thesis were received with the help of cumulative modern methods by using specific chromogenic substrates. It was discovered that while modelling experimental models of diabetes mellitus with the help of streptozotocine and aloxane in the system of “elastase/elastase inhibitors” takes place towards the increase of protease activity. Indicated changes were determined in blood serum, aorta and pancreas tissues in both models of diabetes. In case of aloxane diabetes mellituschanges in elastolytic system assume more In case of aloxane diabetes mellitus changes in elastolytic system assume more considerable character that is explained by compensated ketoacidosis development. While development of diabetes significant changes take place in composition of elastase inhibitors – in case of streptozotocine model, mainly, due to decrease of alpha-2-macroglobulin (in blood serum and aorta tissues), and in case of aloxane modeldue to alpha-1- proteinase inhibitor in all materials under study. Partially it could be us explained by a different toxic action of diabetogenic substances. It was determined that imbalance between component of elastolytic system takes place at early stages of diabetes modelling and is increased in dynamics of experiment. In case of streptozotocine model of diabetes mellitus in aorta tissues of rats imbalance of “trombin/trombin inhibitors” system takes place towards the increase of proteolytic enzyme. The basic connecting links between the imbalance in investigated proteolytic systems and the development of arteriosclerotic changes in vascular wall.