Lubomirskaya V. The functional state of the thymus lymphoid population at the dynamics of diabetes mellitus and its modulation with neuropeptides administration

This dissertation devoted to studying functional state of lymphoid population in thymus and endocrinal cells in experimental diabetes mellitus, and also to correction springing immune disruption with a hypothalamic neuropeptides. Endocrinal part of pancreas was an object of guide. The modern methods as a morphometrics, gistohemistry and immunocytochemistry methods, and also mathematics classification and statistics analysis used for solution this task. The small lymphocytes were predominant type of cells, and the lymphoblasts were the least type and the most concentration was in the subcapular zone. Immunofluorescence revelation nuclear antigen of proliferation cells PSNA with a monoclonal antigens are point to proliferation activity lymphocytes in a rats thymus as in a cortex so in a medullar zones. The density index of population CD134-immunopositive lymphocytes was growths only by the end of the 4-th week development of EDM. The phase change of proliferation activity was observed in a dynamic of EDM: the growth at the 1-st and the3 4-th weeks, and the depression at the 2-nd and the 5-th weeks. The 10-days of entering the NPY to diabetes rats brought to growths the density index of population lymphocytes of thymus at the 4-th week of disease that were growth with the small and medium lymphocytes in the deep cortex and in the subcapsular zones. The effect is bigger with an intracereberoventricular entering the NPY than with an intraperitoneal. After the 10-days entering of oxytocin that was started at the 4-th week of EDM resulted in growths the density of population lymphoid cells of thymus. It was with the growth number of small lymphocytes. The intracereberoventricular entering this neuropeptides were resulted in growth the number of lymphocytes in the medullar zone of thymus only.