Melnik Y. The free radical lipid peroxidation and activity antioxidant system in human and animals ixsposed to lead

The dissertation is devoted to the improvement of several parameters of prooxidant-antioxidant balance and spectral structure of the subfractional macromolecular composition of blood plasma, which were registered by the method of laser correlative spectroscopy in rats and subjects exposed to lead compounds. This research showed that lipid peroxidation can be increased or decreased depending on lead exposure.Our investigation showed decrease in activity of lipid peroxidation in manufacture workers exposed to lead and agriculture machine operators, cultivating soil contaminated with lead. That was connected with rise of the total antioxidant system by means of chemiluminescence of blood plasma and accompanied by functional tension in the antiperoxide link of the antioxidant system. Discord of the lipid composition and decrease of the easily oxidative lipid fraction indicated increase of lipid resistance to peroxidation. Lipid peroxidation was activated in the patients with chronic lead intoxication. Activation of free radical lipid peroxidation was characterized by ramification of that process in blood plasma causing integral antioxidant insufficiency of the organism. Activation of lipid peroxidation, which leads to the formation of TBARS, is a variety of pathological states and has been proposed as a mechanism of the aging processes. The integral humoral homeostasis state was registered by the method of laser correlative spectroscopy (LCS), that was confirmed by the qualitative difference in structure of the subfractional macromolecular composition of blood plasma. The results of the analysis are represented in the form of classification map and histogram. True criteria of difference between two compared groups are distribution of functional particles in each group (%). The analysis of laser correlation spectrums structure of groups of rats (lead, manganese, control) and human groups (control, workers contacting with lead, non-work patients with chronic lead intoxication) allowed detecting high plasma homogeneity in distribution of functional particles in each group. The diagnostic procedure are based on a comparison of the individual peculiarities of blood plasma functional particles for each examined patients to persons in the standard groups (control, workers contacting with lead, non-work patients with chronic lead intoxication) and determining the degree of their similarity, expressed in percentages. Investigations demonstrated the possibility of using of the determination of prooxidant-antioxidant balance and structure of spectrums of the subfractional macromolecular composition of blood plasma as a tool for revealing in certain occupational population persons with a high risk of exposure to of lead compounds.