Oliynyk O. The effect of anion transport inhibitors and membrane modifiers on posthypertonic lysis of erythrocytes

Thesis is dedicated to the study of the effect membrane and cytoskeleton modifiers, anion transport inhibitors and medium composition on the erythrocytes sensitivity to posthypertonic lysis of erythrocytes. The chemical modification of erythrocytes was performed using cell treatment with IAA, PCMB, N-EM, hemin. The modification of intracellular ionic composition was performed using ionophor nigericin. Ionic transport modification was carried out by anion transport inhibitors (DIDS, dipyridamole). Isolation medium contained the sodium salts of chloride, pyruvate, sulfate, citrate and succrose. Obtained results testify that erythrocytes stability to posthypertonic lysis depends on composition of rehydrating medium, that is stability depends on the anions ability to penetrate into cells, when recovery of cell volume occurs. It has been shown that ion redistribution during the rehidratation phase is responsible for erythrocytes sensitivity to posthypertonic lysis of erythrocytes. It has been found thatmembrane modification by IAA/PCMB does not change the sensitivity of erythrocytes to posthypertonic lysis in cytrate and succrose medium. It has been shown that hypertonic treatment does not break the transport function of erythrocyte anion carrier - band 3.