Rogova O. Physical mechanisms of complexation of biologically active aromatic compounds with DNA fragments of different secondary structure

The aim of the work was to study molecular mechanisms of complexation of biologically active aromatic compounds with different structure of chromophore and side chains with DNA fragments of different secondary structure. Methods of research are 1D and 2D NMR spectroscopy and molecular modeling. Complexation of synthetic phenoxazone compound ActII with tetranucleotide 5'-d(TpGpCpA), antitumor antibiotic daunomycin with hexanucleotide 5'-d(GpCpApTpGpC) have been investigated. Comparative analysis of interaction of ethidium bromide with heptanucleotide d(GpCpGpApApGpC), capable to form a hairpin structure, and a single-stranded tetranucleotide d(GpApApG), being a component element of the heptamer sequence, has been made. Analysis of the results enables to conclude that single-stranded non-self-complementery oligonucleotides of the same nucleotide content as in the hairpin loop may be used as model systems for investigation of complexation of aromatic ligands with hairpin structures in solution. Field ofapplication - molecular and medical biophysics.