Object of investigation: DNA, ligands of DNA, correlation between ligands? structure, its DNA-binding properties and biological activity. Aim of investigation: the design, synthesis and studying of properties hydrazine-containing bisinterlexines with acridine, fluorene and anthracene as intercalating fragments. Methods of investigation and appliances: Organic synthesis, NMR-spectroscopy, spectrophotometry and spectrofluorimetry, polymerase chain reaction (PCR), anty viral screening, an estimation of interferon inducing abilities. Theoretical results and their novelty: The assumption according to which presence at compounds intercalating properties allow to expect from them high interferon inducing activity is confirmed. It is shown, that increase of flexibility and lengths of linker results in increase of anty viral activity. By spectrofluorimetry it is shown and in the test of inhibition PCR it is confirmed, that 2-{[2,7-bis-(2-(diethylamino)ethoxy)fluo rene-9-ylhydrazinоcarbonylmethyl]amide}-6-hyd razinоcarbonylmethylamide pyridin-2,6-dicarboxylic acids is the bifunctional combilexine and [2,7-bis-(2-(diethylamino)ethoxy)fluo rene-9-ylhydrazinоcarbonylalkyl]amides pyridin-2,6-dicarboxylic acids - are trifunctional bisinterlexines. Ability of acridone's, 9-formylacridine's and 9-formylanthracene's aminоacetylhydrazones to intercalate in DNA is shown. It is established, that 9-formylacridine's derivatives are most affine in the investigated series. Practical results and their novelty: The improved methods of 9-chlor- and 9-methoxyacridine obtaining are developed, for synthesis acridinylhydrazine's mono- and bis-derivatives advantages of use 9-methoxyacridine are shown. The new lexitropsin-similar bishydrazides and their bis-acridine, bis-fluorene and bis-anthracene derivatives are synthesized and their DNA-binding properties and inhibition of DNA polymerization ability are investigated. The method of primary selection of nucleotropical preparations in the test of inhibition polymerase chain reaction is developed. Among the new acridine?s, fluorene?s and anthracene?s derivatives highly active anty viral agents and effective inductors of a- and b-interferon are found. Subject and the degree of introduction: publications, reports at the scientific conferences, using the results of work in the scientific practice of Scientific Research Institutes of Ukraine. Efficiency of introduction is ensured by the development of the synthesis methods of the novel hydrazide derivatives of acridine, anthracene and fluorene. Sphere of use: chemistry, medicine.
