Kozlova Y. Studing the effect of cryopreservation factors on cells of hemopoietic system under development of autoimmune diseases

The research object. Cryopreservation regimens with variuos concentrations of cryoprotectant and rate of freezing of bone marrow cells of healthy animals and with autoimmune diseases The research aim. Study to the effect of physical and chemical factors of cryopreservation on morphological and functional characteristics of bone marrow cells of animals with different autoimmune diseases Research medods: hematological, immunological, biochemical, morphological, histological methods and those of culturing bone marrow cells in vitro and in vivo. The thesis is devoted to studying the effect of peculiarities of the realized during cryopreservation physical and chemical factors, such as pH and medium osmolarity, cryoprotectant type and concentration, cooling rate on morphofunctional properties of bone marrow cells of healthy animals and those with adjuvant arthritis as well as experimental allergic arthritis in vitro and in vivo. There are demonstrated basic differences of initial functional status of bone marrow cells for healthy donors and those with autoimmune diseases, as well as its changes under similar cryopreservation conditions. The "optimum" of colony forming activity of hematopoietic precursors of healthy animals and those with autoimmune diseases is found to be realized when cryopreserving bone marrow cells under various regimens. There is established the "restoration" effect of the impaired by pathology function of bone marrow hematopoietic precursors of various differentiation level for animals with autoimmune diseases as a result of certain cryopreservation conditions (different concentrations of cryoprotectant and freezing rates).