Somov O. Energetic state of liver during cold storage and normothermic reperfusion

Thesis is dedicated to the study of energetic state of liver during cold storage and normothermic reperfusion. It has been shown that sucrose-based solution (SBS) is able to preserve ATP level of liver and does not yield to commonly accepted University of Wisconsin solution (UW). 1-hour cold storage in the UW causes accumulation of potassium ion in mitochondria, which is retained during 24-hour cold storage. Normothermic reperfusion after 24-hours equalizes the difference between two solutions because of mitochondrial potassium ion release in the UW-preserved livers. ATP-synthase inhibition and increase of olygomycin-insensitive activity are the main reasons of respiration rate decrease after 24-hour cold storage in the two preservation solutions. Albumin that is a compound of the SBS has a protective effect and prevents mitochondrial uncoupling after 24 hour cold storage. Key words: liver hypothermic storage, normothermic reperfusion, isolated mitochondria, oxidative phosphorylation, energy metabolism.