Shkotova L. Development of amperometric biosensors for lactate, ethanol and glucose analysis in wine and must.

The analytical characteristics and effectiveness of different amperometric transducers have been investigated in detail. The methods of immobilization of lactate oxidase, alcohol oxidase and glucose oxidase on the amperometric transducer surface, different from technological point of view, have been studied; the universal immobilization method for all oxidases has been chosen. The ways of improvement of main analytical characteristics of the biosensors developed have been determined regarding their adaptation to industrial conditions. The laboratory prototype of amperometric enzyme sensor system for lactate, ethanol and glucose analysis has been developed. The concentrations of these substances in wine must during its fermentation have been determined quantitatively by the sensor developed. Their comparison with standard methods demonstrated good correlation. The area of application is food industry.