Denysova O. Cryosensitivity of erythrocytes in different mammalian species.

Thesis covers study of erythrocyte sensitivity of different mammalian species (horse, bull, dog) to cryopreservation with cryoprotectants and estimation of structural and functional state of frozen-thawed cells. The results obtained in the work testify to the fact, that DMSO is the most efficient cryoprotectant during cryopreservation for horse, bull and dog erythrocytes. PEO-1500 is capable to preserve cells during cryopreservation, but after transferring such frozen-thawed cells under physiological conditions they undergo damages. After cryopreservation with 10% DMSO in contrast to 15% PEO-1500 the cells preserve morphological indexes. ATP and 2,3-DPG concentrations after cryopresevation the control level keep. Investigation of erythrocyte membrane proteins of animals using electrophoresis method in PAG with protein-cross-linking reagent diamide demonstrated a disorder in their spatial location. Modifications of membrane-cytoskeletal complex are stronger manifested for erythrocytes, cryopreserved under PEO-1500 protection in contrast to DMSO.