Gladkovskaya N. The spectrophotometric analysis of binding of the biologically active ligands to DNA molecules

Complexes of Act ІІ and 6AZC to polyphosphate and DNA molecules with different AT- and GC-content and damaged DNA. To developed a novel technique for processing of the spectrophotometric concentration dependences for ligand-DNA systems. Methods of investigation are spectrophotometric titration in VIS and UV regions, DNA melting in UV regions, numerical calculations of parameters of binding in ligand -DNA systems. A novel technique for processing of the spectrophotometric concentration dependences for biologically active ligand - polyelectrolyte systems has been developed. It is shown that interaction of Act II and DNA with different AT- and GC-content occurs mostly via formation of the hydrogen bonding network, hydrophobic interactions and conformational transitions. The binding process of 6AZC with DNA is endothermic and here binding is determined by hydrophobic interactions. Sphere of use is molecular and radiation biophysics, pharmacology.