Malyarevs'kyj P. Modelling the ischemic brain damage on the organotypic hippocampal slice culture and study neuroprotective action of L-phenylalanine

The dissertation is devoted to investigation the model of the ischemic brain damage on the organotypic hippocampal slice culture and the search for new efficacious agents to prevent neuronal cell damage. The recent results indicate that aromatic amino acids, namely L-phenylalanine (L-Phe), reveal neuroprotective action but protective mechanisms were studied insufficiently. We studied the effects of L-Phe on the viability and cell morphology in rat organotypic hippocampal slice cultures during induced experimental ischemia. Morpho-functional estimation was carried out after oxygen-glucose deprivation (OGD) with different duration and post-OGD cultivation (1, 4 and 24 h). Short-term OGD was chosen for testing L-Phe. L-Phe (100 mkM) was present in the culture medium 30 min prior to OGD, during OGD and reoxygenation. Cell viability and morphological cell changes in CA1 area of hippocampal slice cultures were estimated. Our data showed that the treatment with L-Phe cultures, exposed to OGD, resulted in theincrease cells viability of organotypic hippocampal slice culture and decrease morphological features associated with damage. Thus, L-Phe demonstrates a protective action on hippocampal slice culture in an in vitro ischemia model, suggesting potential efficacy for application of the aromatic amino acids in ischemia related diseases.