Ljoshina L. Cloning root-specific and light-dependent promoters of genes of plants

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0406U004362

Thesis Registration Form

0406U004362.pdf

Applicant for

Specialization

  • 03.00.20 - Біотехнологія

26-10-2006

Specialized Academic Board

Д 26.202.01

Essay

. There are received and investigated the regulator sequences which ensuring root-specific and light-dependent expression of chimerical genes in bacterial and plant cells. The fragment corresponding to the 5'-end sequences of chloroplast ribosomal gene S12 (rpS12) and promoter sequence of the gene small subunit ribulose-1,5-disphosphate of carboxylase (rbcS3A) are extracted and cloned in vectors containing marker genes. The organ-specific activity of cloned promoters has been shown. The gene of metallothioneine was introduced into constructed vector. The increased ability of transgenic plants to accumulation of ions of heavy metals in organ's cells with selective promoter-induced expression of metallothioneine gene has been demonstrated.

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