Salmanova O. Influence of bacterial structural components and glutargin on the functional activity of natural killers in vitro

The thesis is dedicated to the establishment in vitro of the mechanisms of bacterial structural components and glutargin influence on the functional activity of natural killers. For the first time the cytotoxic activity of natural killers under the influence of different concentrations of Staphylococcus aureus peptidoglycans and teichoic acids and Pseudomonas aeruginosa lipopolysaccharides is in-vestigated; it is shown, that bacterial structural components have the dose-dependent influence on cytotoxicity of natural killers - small dozes stimulate it, and high ones - suppress. Production of the tumor necrosis factor alpha, gamma- interferon, interleukins 1beta, 2, 4 and 6 by natural killers, stimulated by bacterial components of different concentration is studied; direct dependence of secretion on the doze and the kind of bacterial component is shown. Features of changes in system of cyclic nucleotides, activity of lipid peroxidation and antioxidant defense system in natural killers after action of different dozes and kinds of bacterial structural components are established. The ability of bacterial components to strengthen the apoptic markers expression CD38 and CD95 on the surface of natural killers is established. The positive influence of glutargin is established on the functional and metabolic activity of the natural killers treated with different concentrations of bacterial structural components. The obtained data are used in educational pro-cess of 2 departments of medical universities of Ukraine.