Object of the study: Dermal and deep burns, donor wounds. Purpose of the research - Improvement results of early surgical treatment of badly burned patients, studying and determination prognoses criteria deficit donor skin resources (DDSR) forming, different methods of burned wounds surgical treatment and development and adoption in practice effective methods of dynamic control reserves of donor resources of autoskin, prophylactics of its deficit growing, development of resource saving cellular technologies of skin coverage renewal. Methods of the research - Are clinical, cytological (wound prints), microbiological; morphological and morpho-metrical methods were used in experiment as well as methods of mathematical analysis and digital analyses data obtained. Practical importance of the received results.For clinical practice a combined method of determination of DDSR in dynamics of surgical treatment was suggested, (patent № 10477 UA). Technology of DDSR growth prophylaxis by means of optimization dermal burns and donor wounds treatment with bio coverage was developed, which helps to decrease the number of complications in 2,3 and 6,6 times compared with traditional treatment and accelerate its healing. Optimised resource saving skin coverage repeir bio technology, combined with early surgical treatment (patent № 5671UA) was developed and was introduced into clinical practice. Presented automicrodermoplastic technology include deficit donor skin resources growth and have an express nature (12-24 h), that meets the requirements of early surgical deep burns treatment. Using these methods helps to individualize treatment and to decrease after-effects frequency and the death level. Scientific novelty of the received results.Quantity criteria of donor skin resources at burned patients with different degree of gravity, and their condition at different treatment stages, were at early and traditional surgical treatment determinated. A new plastic donor material calculation method in dynamics of early surgical treatment was suggested. It was proved, that at early surgical treatment DDSR develops after the first skin restore operation in severe burned patients , that is caused by early beginning , high rate of surgical interventions and depends on the autodermoplastic results, and dermal burns and donor wounds healing. The technology of prevention DDSR growth was developed by means of effective prophylaxis of deepeningdermal burns and donor wounds by using biological coverage. Cytoplastics effectiveness after the primary necroectomy in auto- and allo- models by quantitative (epitelization speed) and qualitative (morpho-functional reparative process features) data were experimentally proved. A new technology of DDSR suppression on the base of resource saving bio technology was developed, quantitative parameters of proliferated epidermal keratinocytes from unit skin coverage area from different anatomic localization was determined on the laboratory stage. Methods of immobilization of epidermal keratinocytes and fibroblasts in vital ksenoderma or reconstructed in vitro derma. Conditions of cell transplant carrying into wound surface its protection from damaging factors of surroundings and wound, also technological parameters and criteria of automicrodermotransplants readiness for clinical use (in 12-24 h). Degree of introduction:Research results are introduced into practice in Kharkov and Sumy burn centers, and also used in studying process traumatology, orthopedic and combustiology КMAPО department. Sphere of application: medicine, surgery.
