Demeshko O. The study of Robinia pseudoacacia leaves biologically active substances and creation of drugs on their basis

The thesis is devoted to the study of biologically active substances in Robinia pseudoacacia leaves and creation of drugs on their basis. The systemic phytochemical and pharmacological research of Robinia pseudoacacia leaves has been carried out. The quantitative composition and qualitative content of different groups of biologically active substances have been studied. 28 individual substances has been isolation from the leaves. Their chemical structure has been determined on the basic of chemical-physical properties of the obtained substances and products of their chemical changes, date of UV, IR, PMR-spectroscopy, in comparison with the reliable samples. 5 hydroxicinnamomic acids, 6 coumarins, 17 fla-vonoids have been identified. Lipophilic fraction has been obtained and its qualitative composition and quantitative content of biologically active substances have been studied.Technology of the dry extract named "Flavacetin", "Liquid extract of Robinia leaves", lipophilic extract and polysaccharide complex from the Robinia pseudoacacia leaves has been worked out. On the base of pharmacological research it has been established that "Flavacetin" is not toxic, has hypoazotemic, hepatoprotective, anti-oxidative and anti-inflammatory action; "Liquid extract of Robinia leaves" has hy-poazotemic and anti-oxidative activity; lipophilic extract has moderate reparative action; polysaccharide complex has haemostatic and anti-inflammatory activity. The dynamics of phenolic compounds accumu-lation in the Robinia pseudoacacia leaves has been studied and optimal terms on their harvest have been determined. For the purpose of creation of AND the main anatomic-diagnostic characteristics of Robinia leaves have been determined. The following draft sets of AND for the medicinal plant drugs "The leaves of the Robinia pseudoacacia", "Liquid extract of Robinia leaves" and information letter "Optimal terms of harvest of medicinal plant drugs - the Robinia pseudoacacia leaves".