Oseychuk O. Features of peroxidative oxidation of the phenolic compounds

Horseradish peroxidaze - the catalyst of oxidization of phenolic pollutants. Research of influencing of structure of phenolic pollutants and terms of their bioconversion in presence free and immobilizet peroxidaze on the degree of their deleting from water solutions. UF-, IR-spektroscopy, mass-spektrometry, elektrophorezis in PAAG, computer analysis. The conditions of the phenolic compounds series peroxidative oxidation were optimized. It was determined , that Ca2+ and Mn2+ ions, water soluble polymers (PVP, PEG-6000) and BSA exerts stabilizing action on peroxidase activity, promoting the enhancement of the bioconversion degree of phenolic pollutants studied. With a help of QSAR and PLS QSPR methods the statistical models are obtained, which allows to predict the degree of transformation and reactivity of the new phenolic substrates. Methods of peroxidase immobilization were worked out, using as a matrice the carrageenan from the Black Sea, poly-N-vinylcaprolactam and hydrate cellulose membrane "Diacell"; the highly active and stable enzyme preparations of repeated usage in reactions of phenols oxidation either in aqueous, or in organic media, are obtained. Sphere of use - bioorganic chemistry, biotechnology.