Tsupykov O. The reaction of hippocampal glial cells to ischemic brain injury

The thesis deals with the analysis of qualitative and quantitative changes of hippocampal glial cells after transient forebrain ischemia and the neuroprotective effect of water-soluble flavonoid quercetine. Mongolian gerbils were subjected to global forebrain ischemia by bilateral occlusion of common carotid artery during 7 min. Hippocampal CA1 area was examined 3, 7, 14 and 28 days after ischemia-reperfusion. It is known, that ischemic brain injury causes transient locomotor hyperactivity in experimental animals. The morphology of hippocampal CA1 area of ischemic hyperactive animals was analysed. GFAP (astrocyte marker)- and Iba-1 (microglia marker)-immunohistochemistry was performed in hippocampal sections. Immunopositive cells were counted in every layer of CA1 region. GFAP- and Iba1-immnunoreactivity increased gradually in CA1 area after ischemia-reperfusion. The number of stained cells reached a maximum at day 14, while from day 14 to 28 glial activation was found to decrease. In addition, the effect of quercetin on the viability and morphology of hippocampal CA1 area cells was studied after induced ischemia-reperfusion. Quercetin assisted in decreasing delayed neuronal death and reducing reactive gliosis after ischemic injury. Thus, quercetin demonstrates a neuroprotective effect on hippocampal cells, suggesting its potential efficacy to treat ischemic brain injury.