Medvedovs'ka M. Тechnological factors influencing on obtaining and culturing of totipotent cells in mice

Рrimary technological factors influencing on obtaining and culturing of mouse totipotent cells are analyzed. Methods and conditions of obtaining and long-term culturing of mouse em-bryonic fibroblasts for their use as feeder monolayer for animal totipotent cells development are specified and perfected. Methods and conditions of effective development of embryonic stem cells precursors are determined. Simplified method of trypsinization of mouse ES-like cells and embryonic fibroblasts is proposed. Possibility of long-term culturing of mouse undifferentiated ES-like cells is evidenced. Modified method of mouse totipotent blastomeres culturing to in-crease an identical genetic material is represented. Illustrative material is collected to show step by step all studies of mouse embryonic fibrolblasts, ES-like cells and totipotent blastomeres de-velopment in culture.