Revenko O. Influence of cryopreserved embryonic somatic cells on skin and cartilage conditions after a traumatic damage in rats

Dissertation thesis is dedicated to the study of primary suspension isolation and cryopreservation of rat somatic embryonic cells (SEC) and elucidation of their influence on regenerative process under condition of experimental skin burn and mechanical damage of articular cartilage as individual types of connective tissues. Cryopreservation under protection of 10% DMSO and 10% of embryonic serum by three-stage freezing program and thawing at 37°С yielded not less than 60% viable cells of SEC primary suspension. Administration of freshly isolated and cryopreserved suspension and to a lesser degree lethally cryopreserved SEC in thermal injury area assisted in chondroblast and fibroblast proliferation, accelerated granulation tissue formation, which was accompanied by leucocytic infiltration decrease and facilitated early skin regenerative process. Application of primary SEC suspension in animals with thermal injury resulted in fast normalization of peripheral blood indices (hemoglobin level, erythrocyteand leukocyte number) and intercellular matrix connectivity tissue compounds (collagen, non-colagen proteins, and glycosaminoglycan). Effect of SEC depends on cell viability: freshly isolated cells recover damaged tissue rather faster than cryopreserved ones. Positive effect of lethally cryopreserved SEC on mechanically damaged cartilage and derma regeneration indicates the role of biologically active substances in implementation of the SEC effects.