Alekseyeva L. Dynamics of changes in erythrocytes distribution on sphericity index in hypertonic solutions of sodium chloride

The dissertation covers an approving of a new original method for determining density of erythrocytes distribution on sphericity index and its utilisation in cryobiological investigations. The characteristics obtained by elaborated method was shown to be sensitive, informative parameter for quantitative estimation of erythrocyte population state. The obtained numerical values of sphericity indices for distribution centres in red blood cell populations of healthy adults comply to the literature data on volume and cell surface for these cells. During directed change of erythrocyte shape by using chlorpromasine the curves for density of erythrocyte distribution on sphericity index reflect adequately the population by its surface-volume ratio. When exposing the red blood cells in hypertonic media their state changes depending on exposure duration and medium hypertonic rate. In the media with mild hypertonia (0.4 and 0.8 M NaCl) in the first 30-90 sec of exposure the number of cells within the low values ofsphericity index (P=1.0-1.3) diminishes. When increasing the exposure duration up to 3-10 min the number of cells with low values of sphericity index increases, and this is the reason of rising of cell sensitivity to following cooling. Increasing of a part of erythrocytes with low values of sphericity index during exposure in 0.4 and 0.8 M solutions of sodium chloride is associated with potassium ions outflux from the cells. During exposure in 1.2 and 2 M sodium chloride solutions significant outflux of intracellular potassium became the main reason, controlling the red blood cell distribution on sphericity index after further placing them into isotonic medium.