Rogova O. Role of microtubule organizing centers in resistance to isopropyl-N-phenylcarbamate mutants of Nicotiana sylvestris L.

By in vitro selection using gamma-irradiation the N. sylvestris mutants resistant to isopropyl-N-phenylcarbamate (IPC) - herbicide from phenylcarbamate class - have been obtained. IPC-resistance of mutants was confirmed by tests on an ability of their leaf explants to regenerate plantlets, and of their callus cells to survive on media with selective concentration of IPC; and also by genetic, morphometric, cytological and immunofluorescence analyses. It was established that aсquired trait of the resistance is inherited in F1 and F2 generations of mutants as dominant nuclear trait. It was shown that formation of tripolar spindles of division and abnormal phragmoplasts simultaneously with disruptions of distributing of gamma-tubulin is the result of fragmentation of microtubule organizing centers (MTOCs), that confirms influencing of this herbicide only on MTOCS of plants. It was established that appearance of several apoptotic disturbances with subsequent depolymerization of microtubules in the cells ofN. sylvestris plants in reply to treatment of nocodazole is direct proof of influence this benzimidazole on microtubule. On the basis investigations it were established that changes in the structure of protein complex MTOCs in the cells of mutant lines N. sylvestris is a basics of IPC-resistance formation.