Voloshyna I. Development of the technology of surface active substances synthesis by oil-oxidizing bacteria

Object - oil oxidizing bacteria Acinetobacter calcoaceticus K-4, Nocardia vaccinii K-8, Rhodococcus erythropolis EK-1, Mycobacterium sp. K-2, Rhodococcus sp. N 1, Rhodococcus sp. N 2 and Pseudomonas sp. PS-27, microbial surface active substances (SAS) aim - development of surface active substances technology with oil oxidizing bacteria using; methods - microbiological, biotechnological, physical-chemical, chemical, mathematical; novelty - it is selected Rhodococcus erythropolis EK-1 - a highly active producer of substances with emulsifying and surface-active properties. It is established conditions of growth R. erythropolis EK-1 on the substrate with ethanol and hexadecane. In structure of synthesized R. erythropolis EK-1 SAS are found glycolipids, phospholipids and neutral lipids, with polysaccharide - protein substances. It is established dependence of oil pollution water clearing degree by immobilized R. erythropolis EK-1 and Nocardia vaccinii K-8 cells from speed of water delivery, a level of aeration and presence of biogenic additives. Result - development of the technology of surface active substances synthesis by oil-oxidizing bacteria. It was established the opportunity of using microbial surface-active sub-stances and immobilized R. erythropolis EK-1 cells on claydite for degradation of oil contaminations. Sphere of use - biotechnology.