Mel'nychenko S. Isolation and characterization of insulin-binding protein factors in blood of diabetic patients

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0408U003562

Thesis Registration Form

0408U003562.pdf

Applicant for

Mel'nychenko Svitlana Vitas’kivna

Specialization

14.01.14 - Ендокринологія

Date of defense

20-05-2008

Specialized Academic Board

Д 26.558.01

STATE INSTITUTE "V.P. KOMISSARENKO INSTITUTE OF ENDOCRINOLOGY AND METABOLISM OF THE NATIONAL ACADEMY OF MEDICAL SCIENCE OF UKRAINE"

Essay

Object: insulin binding protein substances in human serum. The aim of work: to separate and characterize insulin binding proteins (IBP) in human blood serum; to investigate the most important IBP – anti-insulin antibodies (AIA) in blood serum of patients with diabetes mellitus (DM); to develop a method of heterogenic enzyme-linked immuno-sorbent assay (ELISA) for anti-insulin antibodies assay and to create a test system "ИФА-АТ-инс". Methods of research and equipment: affinity chromatography, ELISA, radio-immune assay (RIA), polyacrilamide gel-electrophoresis (PAAGE); ELISA-analyzers “Multiskan EX” и "Stat Fax 3200", liquid chromatograph "Bio Rad". Theoretical and practical results: methodic approaches to separation of insulin-binding protein substances from human blood serum were developed, and efficacy of step-by-step affine chromatography in investigation of IBP in diabetic patients’ blood serum were stated, that would allow to use a differentiated approach to understanding of mechanisms of insulin resistance development in some cases. Novelty: it was shown that step-by-step affine chromatography fractionating of healthy subjects’ and diabetic patients’ blood serum allows to reveal similar fractions of IBP which differ in protein content; insulin-binding serum substances include both AIA and non-immunoglobulin proteins, and equal volumes of patients’ and donors’ serum content different quantities of total IBP so as non-immunoglobulin proteins. Degree of introduction: the results can be used in diagnostics and treatment of DM, and in prediction of DM development. Area of using: endocrinology; clinical and laboratory diagnostics.

Thesis supervisor

Korpachev Vadym Valerievich

Official opponents

Кравченко Віктор Іванович
Кучмеровська Тамара Муратівна

Files

Автореф--Автореферат.doc

Автореф--Обложка автореферата.doc

Дисертація--Glava 1.doc

Дисертація--Glava 2 i 3.doc

Дисертація--Glava 4 i 5.doc

Дисертація--Glava 6.doc

Дисертація--Prakticheskie rekomendacii.doc

Дисертація--Spisok literatyri.doc

Дисертація--Vvedenie.doc

Дисертація--Zakluchenie.doc

Дисертація--ВИСНОВКИ.doc

Дисертація--Мat. i metod.doc

Дисертація--Огляд літератури.doc

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