Grigoryan K. Pathogesis of renal dysfunction under alloxan dibetes in rats with different activity of eNOS and protenkinase С

Object of investigation - morphofunctional state of kidneys, diabetes mellitus, intracellular signaling systems; aim - investigation of eNOS-protein kinase G system and protein kinase C role in pathogenesis of renal dysfunction under alloxan diabetes; methods - physiological, biochemical, morphological, statistical; newness and results - it first shown that reserve power of eNOS determines the efficacy of compensatory reactions in kidneys in diabetes; in normoreactive rats the activation of eNOS- protein kinase G system was detected during 1st month of diabetes, that optimized microcirculation in diabetic kidneys during hypertrophy, accompanied with increase of GFR and fluid reabsorption in proximal tubules, thick ascending limb of Henle's loop and collecting ducts; in hyporeactive rats to the end of 1st month the decrease of eNOS- protein kinase G system with activation of protein kinase C and phosphodiesterases took place, that led to mesangial expansion, proteinury, alteration of peritubular microcirculation , development of tubular dystrophy, leukocytes infiltration and fibrogenesis; inhibition of protein kinase C prevented these changes due to optimization of intracellular signaling system eNOS-protein kinase G state; introduced - in practice of Institute of urgent and recovery medicine surgery AMS of Ukraine, SII of traumatology and orthopedy of DonNMU, and in educational process at departments of pathophysiology of medical universities in Odessa, Zaporozhe, Kharkov, Lugansk and Crym; scope of use - diagnostic and medical process of nephrological, endocrinologyical and therapeutic department, educational process and the departments of pathophysiology at Medical Higher Schools.