Trotskiy P. Influence of deep freezing bovine oocytes on their subsequent development in vitro

Object - is processes of cryopreservation, оо- and embryogenesis of in vitro. The purpose - is development of technological terms of ultrafreeze of bovine ооcyte-cumulus complexes, estimation of viability and subsequent development of frozen-thawed gametes in the conditions of in vitro. Methods - biotechnological, cryobiological, cytogenetical, morphological. Novelty - first developed and approved methods of receipt, cytomorphological estimations, cryopreservation, maturation and fertilization in vitro frozen-thawed and matured bovine gametes, receipt from them of embryos of cattle. The different sensitiveness of bovine ооcyte is set to the low temperatures depending on the stage of their meiotic maturation. Certainly efficiency of different methods of leadingout of cryoprotectors after unfreezing of bovine ооcyte, application of paillete of different diameter and variose concentrations of cryoprotectors in the general volume of vitrification solution at cryopreservation of ооcyte. It is well-proven that most suitable for freezing it is been bovine gametes with dense multi-layered cumulus. Subsequent development of in vitro embryos, got from frozen-thawed ооcytes, must be conducted on monolayer of cumulus or granulese cells.