Dzhuran B. Influence of activators of ATP-dependent potassium channels on electro-physiological properties of smooth muscles of ureter of mammals at an autoimmune injury.

The thesis is devoted to research of action of new fluorine-containing activators of KATP - channels on the electrical and contractile reactions of smooth muscles of human and guinea-pigs ureter at normal conditions and at an autoimmune injury, determined by a acute obstructive pyelonephritis. It is set that in smooth muscles of ureter of mammals KATP-dependent channels function, activity of which is modulated by new fluorine-containing compounds (substances PF - 5 and PF - 10). It is shown that they have the dose-dependent inhibitory effects on contractile and electrical activity of ureter smooth muscles of human and guinea-pigs. For the first time it is shown that they have binary action on smooth muscle cells, that is expressed by activation of KATP-channels and blocking of L - type Ca2+ channels. It is set that in patients under an obstructive pyelonephritis autoantibodies are detected in blood which in high titres react with the contractile proteins of myocytes of ureter of human and guinea-pigsand influence on functional activity of the isolated strips: cause the increase of frequency and amplitude of spontaneous phase contractions, action potentials, depolarize the membrane of myocytes, strengthen the tonic component of contraction. For the first time it is shown that in vitro in the conditions of the induced autoimmune injury of ureter myocytes of mammals by the autoantibodies the usage of activator of KATP - channels (compound PF - 5) significantly diminished the activating effect of antibodies on contractile and electrical activity of smooth muscle cells and prevented forming of the tonic components of isometric contractions, thus showing a protector action which can have practical importance for application at disturbances of the urodynamic of different genesis including also an autoimmune component.