Nizhelska O. 3. Influence of microwave irradiation on the Saccharomyces cerevisiae yeast culture, bacteria Escherichia coli and green alga Dunaliella viridis

The same biological subjects may give different responses (or zero reaction) after microwave exposure with certain frequency. These phenomena need more deep analysis to define the circumstances influencing a sensitivity of the cells to the weak microwaves. The original method of yeast S. cerevisiae growing and exposing was described. The main difference from other works is that method of yeast S. cerevisiae growing and exposing was yeast culture should be cooled and synchronized before microwave irradiation. The concentration of the cells were counted by microscope through certain time. The frequency dependence of yeast growth were obtained. Time dependence of biological effect was significally non-linear.It was shown that irradiated samples had grown more quickly than the controls and reproduced this feature in the next generations of the cells. It points that genetic mechanisms were implicated here. In the work the suspensions of motile cells – bacteria E.coli and alga D.viridis were studied by quasi-elastic light scattering in real time. It was proved that a gradient of microwave EMF in the suspension caused a directed collective motion of bacteria E.coli and green alga. A heat effect was excepted by special checking. The new result was the active cells at their optimal conditions had a low sensitivity to microwave EMF. The cells with slow metabolic processes due to cooling and poor medium revealed a high sensitivity to microwave exposure. Thus the populations of well known cells like yeast, bacteria E.coli and green alga could be the test-subjects for estimation of biological activity of weak microwave fields.