Matvieieva N. Construction of transgenic plants Compositae and Solanaceae families

The thesis is devoted to construction of Solanaceae and Compositae plants (Solanum rickiі, Solanum tuberosum, Lycopersicon peruvianum, Lactuca sativa, Cichorium intybus) with transformed nuclear or chloroplast DNA. For this purpose the methods of somatic hybridization, biolistic and Agrobacterium-mediated transformation have been used. The biolistic method was used for Solanum rickii chloroplast transformation with aadA gene. The hy-brid plants with transformed plastids were regenerated after PEG fusion of chlorophyll-deficient L. peruvianum leaf mesophyl protoplasts and leaf mesophyl protoplasts from the S. rickii plants. Transgenic plants of Lactuca sativa L. with genes cod-ing synthesis of tuberculosis antigens have been obtained by Agrobacterium-mediated transformation. The efficient method for plant regeneration and Аgrobacterium-mediated transformation with interferon gene has been developed for Cichorium intybus L.