Parnikoza I. Rauwolfia serpentina cell population dynamic in different in vitro grow conditions

Rauwolfia serpentina Benth. tissue culture cell population structure for passage and circadian levels have been studied for cells with various DNA content in the nucleus and the nucleolus area in the nucleus under conditions with different type (solid/liquid) and contents of nutrient medium. A wide variety of DNA content and nucleolus area in the nucleus have been shown in researched conditions of cultivation. Dried biomass, indoline alkaloids, tracheides accumulation production indеxes have been turned out to be supported by different combinations of cell contribution with certain nucleolus size: 1,0-2,9С and 3,0-6,9С (for К-27(10S) and К-27(5S)) or 3,0-8,9С (for К-27(Rzh). Circadian dynamics of these cell-subpopulations functionally-important groups have been shown to be conditioned by proliferation dynamics. Proliferation in dependence of time intervals alteration have been shown to make contribution to cell dynamics with different DNA amount and nucleolus area and these circumstances have been shown to define the individual producing features at a passage level.