Sobolta A. Fasciolosis of catlle (cytogenetic investigations under influence of fasciolocide preparations)

The thesis is devoted to the mechanisms and relations in the system owner-parasite on the cytogenetic level during appearance of fasciolosis of catlle and influence of fasciolocides preparations. There was carried out the eрizootic monitoring as to fasciolosis in different farms of Western Ukraine. There was investigated frequency of genome and chromosomal changeability in somatic cells of lymphatic organs of the owner as a display of cytogenetic changeability in the genome under pathogenetic influence of Fasciola hepatica and was unsteadiness that spontaneous level of pathological mitoses under the influence of biotic, abiotic factors of the environment and automutagenes was very high. Dependency of increase in the level of micronuclear erythrocytes from the degree of invasion was studied. Fasciolocide preparations showed high efficiency. They were the following: combitrem, brontel 10%, brovalzen. They showed no mutagenic action on cows in therapeutic doses.Biological resistance of Fasciola hepatica eggs to antihelmintics was attentively studied and as a result there was worked out biological test of miracidium.There was investigated the influence of fasciolocides on mature Fasciola hepatica in vitro, and there action on cenogenesis, mitos and meios, which may be the reason for cytogenetic changes in gamets and appearance of the resistance to antihelmintics.