Zubova O. Structural-functional condition of cord blood cell populations after cryopreservation with the nonpenetrating cryoprotectant PEO-1500

The research object is influencing of the cryoprotectant PEO-1500 and cryopreservation processes on erythrocytes and nucleated cells including stem hemopoietic ones, and cord blood plasma biologically active substances. The aim of the work is studying the structural and functional condition, safety and viability of erythrocytes and different nucleated cell populations including stem hemopoietic ones, and safety of cord blood plasma biologically active substances under cryopreservation with the nonpenetrating cryoprotectant PEO-1500 depending on cooling regimens. The methods of investigation are spectrophotometry, light microscopy, enzymoimmunoassay, PAAG-electrophoresis, flow cytometry. The data obtained were statistically processed by Student and Fisher tests using the program "Stat Graphіcs". It has been established that freezing at the rate of 60 grad/min up to -40 grad before a dip in liquid nitrogen with a preliminary processing with the cryoprotectant PEO-1500 (the regimen CWCB) is an optimal regimen for simultaneous preservation of erythrocytes and nucleated cells including stem hemopoietic ones. Erythrocytes frozen in the whole blood according to the regimen CWCB are characterized by a high safety both right after thawing and after a simulative transfusion. It was discovered that populations of lymphocytes and monocytes were more cryotolerant, while cells from granulocytic series were more sensitive to low temperature influence.