Soldatkin O. Development of bioselective elements of mono- and multisensors for ecological monitoring.

3/ The object - enzymatic hydrolysis of substrates and its inhibition by toxic agents; the aims - development of new bioselective elements of mono- and multisensors for determination of toxic agents such as formaldehyde, heavy metal ions and pesticides; the methods - conductometric and potentiometric methods for investigation of enzymatic systems inhibition by different toxicants; the new approach - in first enzyme formaldehydedehydrogenase co-immobilized with NAD+ and DEAE-dextran in BSA-membrane was used as high-sensitive element of conductometric biosensor for formaldehyde determination. Firstly three-enzyme system including invertase, mutarotase and glucose oxydase was used for creation of bioselective element of conductometric monobiosensor or bioselective element of potentiometric multisensor for determination of heavy metal ions. In first configuration of multibiosensor based on pH-sensitive field-effect transistors and five bioselective elements was developed for simultaneous determination of heavy metal ions and pesticides; the results - two laboratory prototypes of conductometric monobiosensors for direct determination of formaldehyde and inhibitory analysis of mercury and silver ions, and prototype of potentiometric multisensor for toxic agents analysis were created. Such devices can be used for ecological monitoring of different toxicants in water samples; the field - biotechnology.