Oleshko A. Pro- and antioxidant mechanisms of the immobilizing-cold stress and their changes under the influence of the physical factors

The investigation object: mechanisms of ISC and their variation under influence of physical factors. The investigation goal: elucidation of features of action of physical medical factors: US, LILR-IR, PA - on activity of LPO/AOS system in microsomal fraction (MSF) of liver in the conditions of immobilizive cooling stress (ICS). Methods of research: pathophysiological; biochemical determination of spontaneous, NADF-dependent and ascorbate-dependent LPO, biochemical determination of catalase activity, SOD, GR , GP in liver microcosms; macroscopic examination of stomach mucous membrane; statistical.The dissertation work is devoted to the study ground of influencing mechanisms of the physical factors on the activity of the prooxidant and antioxidant systems of hepatocytes of the rats liver in the conditions of the immobilization cold stress model. On the basis of the conducted biochemical complex researches on 375 white not thoroughbred rats the features of action of such physical factors, as ultrasound, low-intensive infrared laser irradiation, medicinal mud were set on the system of LPO/AOS in microsomal fraction of the rats liver in the conditions of the stress model. It has been revealed, that the applied physical factors assisted reducing the broken balance in the LPO/AOS system stipulated by the stress reproducing and intensification of the reparative processes in mucous membrane of stomach. The features of the indicated effect, determined by the type of physical factor and intensity of its influence, are exposed. It allows examining the action of the studied physical factors as the important pathogenic method of the stress-determined damages correction. The degree of implementation: the results of the research are implemented in the educational process and scientific activity at Odessa national Medical University named by I.I. Mechnikov, A.V.Bogatsky Physico-chemical institute of NAS of Ukraine and Ukrainian research institute of medical rehabilitation and balneology.