Ershov S. Osmotic and temperature sensitivity of mammalian erythrocytes

The research object: hypertonic shock and hypertonic cryohemolysis of mammalian erythrocytes. The research aim: there was studied the development of depending on the changes in osmotic and temperature conditions of an environment, as well as in the presence of amphiphilic compound, chlorpromazine. The research methods: microhematocrit method, optical microscopy method, spectrophotometric method, ionometric method. Human, bovine, canine, equine and rabbit erythrocytes were used in the research. It has been revealed that in 4,0 mol/l NaCl containing medium at 37 °C the maximum level of hemolysis was found for human and bovine erythrocytes and the minimum one for those of dog and rabbit. It has been established that at incubation temperature of 0 °C the sensitivity of animal erythrocytes to hypertonic shock is significantly lower if compared with 37 °C. The temperature ranges of the development of hypertonic cryohemolysis of mammals have been determined. It has been noted that yield of K+ ions out of erythrocytes and change of their cellular volume during lasting incubation in electrolyte and non-electrolyte media is not decisive in respect to the peculiarities of the dynamics of hypertonic cryohemolysis of the erythrocytes of all the studied mammals. High antihemolytic activity of chlorpromazine under hypertonic shock and hypertonic cryohemolysis in respect to the investigated cells has been revealed.