Research object: regional raspredilenie RV in the objects of waters of environment on territory of Ukraine, circulation of RV among patients on OKI, processes of adsorption and elyutsii RV natural and artificially synthesized sorbentami. Purpose: to perfect methodical approaches to determination of rotavirusiv man in water and to study the regional features of their distribution in the objects of waters of environment on territory of Ukraine and among patients by sharp intestinal infections, to give molecular genetic description to the selected exciters.. Methods: for implementation of work the classic and modern methods of virology research are used: stationary and rolernoe cultivation in the culture of cages, selection and tipirovanie viruses, determination of infectious title, indication of RV by the methods of unline of gemaglyutinatsii (RNGA), imunofermentnogo analysis (IFA), by rapid tests, method of fluorescent antibodies (MFA) and methods of electronic microscopy. The row of researches is executed with the use of molecular genetic methods: selection of RNK of viruses, polimeraznaya chain reaction with reverse transcription (PLR-ZT), elektroforetichniy analysis, PLR in real time, multiplexed PLR with гибридизационно-fluorescent detektsiey products of amplifikatsii. A statistical method was used on all stages of work for treatment of results of research and determination of authenticity of findings. Theoretical and practical results: consist in the experimental ground of new method of concentration of rotavirusiv from water, improvement of methodical approaches and charts of concentration, suggestion of algorithms sanitarno- virology research by a sewer, drinkable and waters of superficial and underground reservoirs for monitoring of rotavirusnoy infection. Study of features of circulation of rotavirusiv of various genotypes on territory of Ukraine and among patients of OKI, receipt of information molecular genetic description of the selected cultures, including cultures, which have global distribution of P[8]G1, P[8]G2, P[8]G3, P[8]G4, P[4]G2. Novelty: first developed and grounded new method of concentration of RV from water with the use of artificial synthesized and standardized sorbenta of GGMKK, the basic factors of influence are studied on the processes of adsorption and elyutsii RV of man and animals, high adsorption activity of GGMKK is set in relation to RV of man, the optimum parameters of effective adsorption and most effective terms of elyutsii are certain . Distribution of rotavirusiv man of group is set And on territory of Ukraine, certainly their [Р]G-genotypes and санітарно is improved is virology control of quality of water of different type of vodopol'zovaniya and degree of contamination in relation to its epidemic safety. The degree of introduction consists in tom, that the laboratories of virologies of SES of Ukraine were got by the "Methodical pointing sanitary of virology control of quality of objects" of waters of MOZ of Ukraine ratified an order ot 30.06.2007 after № 284. In this normative document sections are written with an author 3,4,5 and 6, charts are presented in which, algorithms sanitary virology research of water of different type of vodopol'zovaniya and contamination, and also methods of concentration and indication of rotavirusiv, selection in the culture of cages and authentication of the selected agent. Sphere (industry) of the use: medicine.
