Borysco P. Ca2+, Н+ -dependent regulation of smooth muscles contraction-relaxation kinetic characteristics and its modulation by reverse effectors

Object: smooth muscles. Aim: applying mathematical and molecular modeling to create the models of the influence on enzymatic and transport activity of smooth muscles’ proteins for certain reverse effectors, including Ca2+ , H+ ions. Methods: theoretical methods of physical kinetics and biophysics (creating differential equations for analysis of complex biological systems), molecular modeling methods and experimental methods for enzyme kinetics. Results: Several mathematical methods, determining kinetic characteristics of reverse effectors’ action (e.g. Са2+ ions, staphylococcus’ protein A, protons, reverse inhibitors) on enzymatic (transport) smooth muscles’ proteins activity are proposed. Novelty: The background of staphylococcus’ protein A action on smooth muscles is proposed according to the designed mathematical model. New optimized mathematical way determining kinetic characteristics of reverse effectors’ action is anticipated. Explanation for linear pH-dependence of the „basal” Са2+ -independent Mg2+ ATP-ase reaction rate is proposed. Mathematical model proposed an explanation for non-typical two-peaks pH-dependence of smooth muscles’ myosin ATP-ase activity. 82 potent myosin ATP-ase inhibitors were selected according to the molecular modeling procedure. It is introduced in an R&D work of two institutes of NAS Ukraine. Field of the use: biophysics, biochemistry, molecular biology, pharmacology.