Stepanskiy D. Influence of selenium on probiotic association aerococcus viridans 167, bacillus subtilis 3 ecsherihia coli v 17

Object of study: containing selenium probiotic microorganism - A.viridans 167, E.coli M 17 and B.subtilis 3. The study aims to assess the impact of the complex salts of selenium on probiotic microorganism: A.viridans 167, B.subtilis 3, E.coli M17, their biological properties and the possibility of introducing selenium in the macroorganism with subsequent translocation to the improvement of probiotics and increase the protective forces of organism. Research methods: microbiological, biochemical, voltammetric method, mathematical and statistical. Equipment: microscope phase-contrast, light microscope, analytical balance, voltammetric analyzer ABA-2 firm "Burevestnik" (Russia). Scientific novelty: Proved, that selenium is actively absorbed by the probiotic and conditionally pathogenic microorganisms. Absorption of selenium by microorganisms leads to an increase in their resistance to oxidants (H2O2, NClO). Established that the translocation containing selenium probiotic microorganisms in the rat organism is an increase concentration of selenium in animals blood and urine, which can contribute to correct the concentrations of selenium in selen deficiency macrooganisms. The obtained data showed no significant antagonism between probiotic Association A.viridans167, E.coli M 17 and B.subtilis 3, which in turn can be used for joint cultivation of these probiotic microorganisms with a view to create a new multiprobiologic substance. Developed a series of indicator medias for visualization of biochemical properties of the studied probiotic organisms. On the basis of the oxidation of lactic acid developed a model for study the antioxidant activity of probiotic microorganisms and biological substrates. The rate of introduction: The methodological recommendations, the information letter, patent number 80892 Ukraine. Scope of application: microbiology (medicine).