Dekina S. Proteolytic enzymes and proteins immobilization on polymeric supports.

The thesis is devoted to study of proteolytic enzymes and proteins immobilization on synthetic and natural polymeric supports. It was shown, that the proteins immobilization in PVA occurs both by complex formation and mechanical entrappment of protein in polymer. As a result of HSA immobilization in PVA were obtained OMI of prolonged action, with quantitative degree of entrappment, stable for a long time, with high adsorbtive capaсity towards acids and alkalies, which is prospective for sorbtive therapy of ocular burns. The substrate inhibition of papain, trypsin and alkaline protease was found, which allowed to recommend usage of 0,35 - 0,9 % casein solution. Joint immobilization of papain and urea had brought to obtaining OMI with 1,2-fold increased proteolytic activity, prolonged action, stable to gama-irradiation and storage, prospective for cornea burn therapy. It was shown, that the developed method of alkaline protease and trypsin immobilization in PVP, modified by polysilicic acid sol, broughts to obtaining water insoluble hydrogel wounds coatings with high proteolytic activity, stable at storage and gama - irradiation, prolonged action. The invented method of protease C immobilization in biogel "Lamidan" had allowed to create textile coatings with quantitative retaining of proteolytic activity, prolonged action and long-term storage for potential usage in wound and burn therapy. The laboratory rules on OMI with papain and urea; the technical code project on immobilized alkaline protease and OMI with papain and urea were drawn up.